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human leukemia inhibitory factor (hlif) peprotech 300-05  (PeproTech)


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    PeproTech human leukemia inhibitory factor (hlif) peprotech 300-05
    Human Leukemia Inhibitory Factor (Hlif) Peprotech 300 05, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human leukemia inhibitory factor (hlif) peprotech 300-05/product/PeproTech
    Average 90 stars, based on 1 article reviews
    human leukemia inhibitory factor (hlif) peprotech 300-05 - by Bioz Stars, 2026-06
    90/100 stars

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    (A) Representative images and quantification of NKX2.1 + , PAX6 + , and OLIG2 + -expressing cells after 1 week of patterning (n = 3 from three independent experiments). Scale bars: 20 μm. (B) Representative images of hMAP2 + and GABA + cells in the brains of Rag2 −/− mouse. Arrows indicate hMAP2 + GABA + <t>inhibitory</t> neurons; Arrowheads indicate hMAP2 + GABA - excitatory neurons. Scale bars: 20 μm.
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    (A) Representative images and quantification of NKX2.1 + , PAX6 + , and OLIG2 + -expressing cells after 1 week of patterning (n = 3 from three independent experiments). Scale bars: 20 μm. (B) Representative images of hMAP2 + and GABA + cells in the brains of Rag2 −/− mouse. Arrows indicate hMAP2 + GABA + <t>inhibitory</t> neurons; Arrowheads indicate hMAP2 + GABA - excitatory neurons. Scale bars: 20 μm.
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    (A) Representative images and quantification of NKX2.1 + , PAX6 + , and OLIG2 + -expressing cells after 1 week of patterning (n = 3 from three independent experiments). Scale bars: 20 μm. (B) Representative images of hMAP2 + and GABA + cells in the brains of Rag2 −/− mouse. Arrows indicate hMAP2 + GABA + <t>inhibitory</t> neurons; Arrowheads indicate hMAP2 + GABA - excitatory neurons. Scale bars: 20 μm.
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    (A) Representative images and quantification of NKX2.1 + , PAX6 + , and OLIG2 + -expressing cells after 1 week of patterning (n = 3 from three independent experiments). Scale bars: 20 μm. (B) Representative images of hMAP2 + and GABA + cells in the brains of Rag2 −/− mouse. Arrows indicate hMAP2 + GABA + <t>inhibitory</t> neurons; Arrowheads indicate hMAP2 + GABA - excitatory neurons. Scale bars: 20 μm.
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    (A) Representative images and quantification of NKX2.1 + , PAX6 + , and OLIG2 + -expressing cells after 1 week of patterning (n = 3 from three independent experiments). Scale bars: 20 μm. (B) Representative images of hMAP2 + and GABA + cells in the brains of Rag2 −/− mouse. Arrows indicate hMAP2 + GABA + inhibitory neurons; Arrowheads indicate hMAP2 + GABA - excitatory neurons. Scale bars: 20 μm.

    Journal: bioRxiv

    Article Title: Co-Transplantation-Based Human-Mouse Chimeric Brain Models to Study Human Glial-Glial and Glial-Neuronal Interactions

    doi: 10.1101/2024.07.03.601990

    Figure Lengend Snippet: (A) Representative images and quantification of NKX2.1 + , PAX6 + , and OLIG2 + -expressing cells after 1 week of patterning (n = 3 from three independent experiments). Scale bars: 20 μm. (B) Representative images of hMAP2 + and GABA + cells in the brains of Rag2 −/− mouse. Arrows indicate hMAP2 + GABA + inhibitory neurons; Arrowheads indicate hMAP2 + GABA - excitatory neurons. Scale bars: 20 μm.

    Article Snippet: The pNPCs were cultured in a medium consisting of a 1:1 mixture of Neurobasal (Thermo Fisher Scientific) plus GlutaMax (Gibco) and DMEM/F12 (Hyclone), supplemented with 1x N2, 1x B27-RA (Thermo Fisher Scientific), FGF2 (20 ng/ml, Peprotech), CHIR99021 (3 μM, Biogems), human leukemia inhibitory factor (hLIF, 10 ng/ml, Millipore), SB431542 (2 μM), and ROCK inhibitor Y-27632 (10 μM, Tocris).

    Techniques: Expressing